منابع مشابه
Rigidity of melting DNA.
The temperature dependence of DNA flexibility is studied in the presence of stretching and unzipping forces. Two classes of models are considered. In one case the origin of elasticity is entropic due to the polymeric correlations, and in the other the double-stranded DNA is taken to have an intrinsic rigidity for bending. In both cases single strands are completely flexible. The change in the e...
متن کاملMolecular rigidity and enthalpy-entropy compensation in DNA melting.
Enthalpy-entropy compensation (EEC) is observed in diverse molecular binding processes of importance to living systems and manufacturing applications, but this widely occurring phenomenon is not sufficiently understood from a molecular physics standpoint. To gain insight into this fundamental problem, we focus on the melting of double-stranded DNA (dsDNA) since measurements exhibiting EEC are e...
متن کاملMaking DNA melting useful.
Featured Article: Wittwer CT, Reed GH, Gundry CN, Vandersteen JG, Pryor RJ. High-resolution genotyping by amplicon melting analysis using LCGreen. Clin Chem 2003;49:853– 60. DNA melting (the denaturation of DNA by heat) is a fundamental property of DNA that can be used as an analytical tool. Classic studies of DNA melting monitored the absorbance near 260 nm as the temperature was increased and...
متن کاملEarly melting of supercoiled DNA.
Denaturing gradient gel electrophoresis (formamide with urea) has been used to study the melting of supercoiled DNA. A linear gradient of denaturant concentration proportional to a 25 degrees C linear increase of temperature (Teff) from the left to the right edge of the gel was created perpendicular to DNA migration. The mobility of supercoiled DNA molecules was shown to drop to the level of re...
متن کاملMelting and unzipping of DNA
Experimental studies of the thermal denaturation of DNA yield a strong indication that the transition is first order. This transition has been theoretically studied since the early sixties, mostly within an approach in which the microscopic configurations of a DNA molecule are given by an alternating sequence of non-interacting bound segments and denaturated loops. Studies of these models negle...
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ژورنال
عنوان ژورنال: Physical Review E
سال: 2016
ISSN: 2470-0045,2470-0053
DOI: 10.1103/physreve.93.052102